In this study, Alkaline protease producing bacterial strains were isolated from various sample using skimmed milk agar. Among the isolates, Strain RE-1 shows highest protease activity which was identified as Bacillus sp. by partial 16S rRNA sequencing. The isolate showed maximum alkaline protease production (89.396 U/ml) at pH 8.0 and 30°C within 24 h of incubation. The enzyme exhibited thermal stability in the range of 40 to 70oC. Specific activity of partially purified enzyme is found to be 0.53 U/mg with 37.8% yield.
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