PRESERVE: adding variable flip-angle excitation to TROSY NMR spectroscopy

We introduce the PRESERVE pulse sequence element, allowing variable flip-angle adjustment in 2D 1H-15N and 1H-13C TROSY-type correlation experiments. PRESERVE-TROSY exploits a remarkable array of up to nine orthogonal polarization-coherence transfer pathways, showcasing the remarkable potential of spin manipulations achievable through the design and optimization of NMR pulse sequences.