Supplementary Figure S6 from Repolarization of Immunosuppressive Macrophages by Targeting SLAMF7-Regulated CCL2 Signaling Sensitizes Hepatocellular Carcinoma to Immunotherapy

(A) Flow cytometry analyses of CD80 and CD206 expressions in THP-1-differentiated macrophages cocultured with the indicated HCC cells. (B) Flow cytometry analyses of MHC-II and CD206 expressions in BMDMs cocultured with the indicated HCC cells. (C) qPCR analyses of M1 and M2 marker genes transcription levels in BMDMs cocultured with the indicated HCC cells. (D-E) Chemotaxis assays showing the effect of supernatants from the indicated coculture systems on the migration ability of THP-1-differentiated macrophages and BMDMs. Scale bar: 100 μm. (F) The correlations of intra-tumoral SLAMF7 expression with CXCL10, CXCL9, and ARG1 expression in TCGA-LIHC cohort. (G) The prognostic prediction performances of SLAMF7 in combination with CD68, CD80, and CD206 for OS in our Fudan LCI HCC cohort. *P<0.05, **P<0.01, and ***P<0.001, Student’s t test. Experiments were repeated three time and results were present as the mean ± SD.