Calibration of non-amplified nucleic acid quantitative fluorometer

This article developed a calibration method to evaluate the metrological characteristics of non-amplified nucleic acid quantitative fluorometers, by studying the working principle of non-amplified nucleic acid quantitative fluorometer. Multiple different types of non-amplified nucleic acid quantitative fluorometers were selected for pre-testing and national certified reference materials are chosen as reference. Critical parameters such as indication error, repeatability, and correlation coefficient are selected as test items. The experimental results shows that, the method proposed and reference materials selected can effectively evaluate the metrological performance of the instrument, which is of great significance for the quality control and quantity traceability of non-amplified nucleic acid quantitative fluorometers.