Proteomics based systematic exploration of the peptidoglycan biosynthesis of Olsenella uli DSM 7084 towards pathogenesis

Abstract The oral microbiota plays a pivotal role in either promoting health or exacerbating disease progression. Within the diverse microbial community, Olsenella uli emerges as a concerning pathogen linked to various endodontic infections. Advancements in next-generation sequencing methods and bioinformatics have begun unraveling the intricate nature of the oral microbiome. Understanding these oral microorganisms opens doors to exploring functional and metabolic changes, offering valuable insights for drug development and targeted therapies. Consequently, our current investigation employs a comparative subtractive proteomics approach to identify potential drug targets within Olsenella uli DSM 7084. This effort unveils eight promising drug target candidates, which undergo thorough assessment for druggability and sub-cellular localization. Furthermore, molecular docking simulations involving these prioritized targets and FDA-approved drugs establish a foundational framework for future researchers, expediting the drug development process aimed at combating infections caused by this formidable pathogen. Our research intends to accomplish precision drug target discovery using an integrated method that integrates subtractive proteome analysis, systems biology, and molecular docking. This method paves the path for more precise molecular docking investigations by enabling a thorough understanding of prospective pharmacological targets.