Inhibition of staphylococcus epidermidis Biofilm Formation in Response to Purified Phytochemical Antimicrobial Materials

A total of 164 clinical specimens were collected from AL-Ramadi city during the period from August 2021 to December 2021 and were taken from surgical wounds, blood ,stones of kidney, eyes, and skin. Specimens were cultured on blood and manitol salt agar for isolation and identification of bacteria .By using biochemical tests revealed that the bacteria was positive for the gram stain, and Catalase test, but negative for the Coagulase, Fermintation of manitol, and Hemolysin tests and by using Vitek compact system GP-2. In this study there are 24 (14.63%) positive samples and 140 (85.37%) negative cultures for staphylococuus epidermidis isolates. Biofilm measured by microtiter plate assay (MtP), 24 isolates (100%) are capable of producing biofilm, 12(50%) of the isolates formed strong biofilm, 10(41.66%) formed moderate biofilm, while 2(8.34%) formed weak biofilm. The inhibitory effects of Gallic acid, Cinnamic acid, Salicin, Linoleic acid, and Erythromycin, and were tested against biofilms of staphylococuus epidermidis. The phytochemical compounds and antibiotics solution significantly inhibited the initial cell attachment of the staphylococuus epidermidis but were less inhibitory at 8 h incubation in preformed biofilms formed on polystyrene surface, the inhibition of staphylococuus epidermidis preformed biofilm was increased with increasing incubation time except for efficacy of Erythromycin. However, there was a synergistic effect between erythromycin with salicin, gallic acid by using Checkerboard technique.