The aim of this study was to reach a well-defined protocol to in vitro propagate of Arganiaspinosaand overcome vitrification phenomenon.In this respect, the highest number of shoot was found at 2.0 mg/l Kin at genotype 3 for multiplication stage.MS medium supplemented with 100 mg/l calcium pantothenate was suited for to eliminate vitrification at all genotypes.For callus induction, MS medium supplemented with 1.5 mg/l NAA when incubated in the light or darkness was favorable to callus induction.MS medium supplemented with 0.5 mg/l GA3 + 1.5 mg/l BA led to increases shoot initiation.
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Holiel et al. (2024) studied this question.
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