Isolation and Identification of a Coumarin Glycoside from leaves of Skimmia laureola.

The leaves of S. laureola were collected from Nachiketa Tal at an altitude of 2450-2500m, District Uttarkashi, Uttarakhand, India. The air-dried and powdered leaves of S. laureola were exhaustively defatted with light petroleum ether (60-800 ). The petroleum free mass extracted with 90% ethanol. The ethanol extract was concentrated under reduced pressure and a suspension of the residue was made with water, and then partitioned sequentially with CHCl3 and n-butanol in a separatory funnel. The butanol layer was separated and concentrated under reduced pressure to give BuOH extract. The nbutanol extract was subjected to column chromatography over Si-gel eluted with different proportion of CHCl3 and MeOH afforded 6-O-β-D-glucopyranoside-2H-1- benzopyran-2-one.