Evaluation of Anti-Helicobacter pylori Activity of Novel Lactic Acid Bacteria

In this study, we aimed to isolate and identify strains of lactic acid bacteria from kkakdugi, to validate their potential use as alternative therapeutic agents against Helicobacter pylori.Eleven strains were identified, including Lactobacillus plantarum, Pediococcus pentosaceus, Lactobacillus brevis, Pediococcus acidilacti, and Leuconostoc mesenteroides.The paper disk method and urease inhibition activity were used to verify anti-H.pylori activity.Without pH adjustment, most strains exhibited an average inhibitory zone of 13.68 mm.However, when the pH was adjusted to 7.0, some strains showed inhibitory zones ranging from 11.65 to 13.15 mm.Nevertheless, upon comparison, it was observed that the antimicrobial activity was higher when the pH was not adjusted.On the other hand, antimicrobial activity against H. pylori G88026 strain was exhibited regardless of the pH.The results of urease inhibition confirmed a significant reduction of approximately 60∼90% in urease activity when the lactic acid bacterial culture supernatant was added.Except for the Lactobacillus sakei strain, the remaining strains exhibited potent urease inhibition activity.This suggests that the lactic acid bacteria isolated in this study could be promising candidates as alternative therapeutic agents against H. pylori.