The study focusing on the development and validation of a high-performance thin-layer chromatography (HPTLC) method for quantifying berberine in pharmaceutical formulations. Various chromatographic parameters were optimized to ensure precise and reproducible results, including mobile phase composition, linearity range, detection wavelength, and spot characteristics. The method demonstrated high accuracy and linearity within the concentration range of 5 - 35 µg/mL, with a determined Rf value of 0.52±0.02 for berberine. Additionally, stability studies revealed that berberine underwent degradation under all stress conditions examined.
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Thakare et al. (2024) studied this question.
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