Therapeutic Potential of Fractions of Globimetula oreophila (Oliv. ex Hook.f.) Leaf Extract Growing on Azadirachta indica against Plasmodium berghei-Infected Mice: An In Vivo Approach

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Therapeutic Potential of Fractions of Globimetula oreophila (Oliv. ex Hook.f.) Leaf Extract Growing on Azadirachta indica against Plasmodium berghei-Infected Mice: An In Vivo Approach † by Dauda GarbaDauda Garba SciProfiles Scilit Preprints.org Google Scholar 1,*, Bila Hassan AliBila Hassan Ali SciProfiles Scilit Preprints.org Google Scholar 1, Bashar BawaBashar Bawa SciProfiles Scilit Preprints.org Google Scholar 2, Abdulrazaq SanusiAbdulrazaq Sanusi SciProfiles Scilit Preprints.org Google Scholar 3, Yahaya Mohammed SaniYahaya Mohammed Sani SciProfiles Scilit Preprints.org Google Scholar 1, Musa Isma'il AbdullahiMusa Isma'il Abdullahi SciProfiles Scilit Preprints.org Google Scholar 4, Muhammad Garba MagajiMuhammad Garba Magaji SciProfiles Scilit Preprints.org Google Scholar 5, Aliyu Muhammad MusaAliyu Muhammad Musa SciProfiles Scilit Preprints.org Google Scholar 1 and Hassan Halimatu SadiyaHassan Halimatu Sadiya SciProfiles Scilit Preprints.org Google Scholar 1 1 Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria 810107, Nigeria 2 National Agency for Food and Drug Administration and Control, Gusau 900288, Nigeria 3 Department of Pharmaceutical Chemistry, Bauchi State University, Gadau 751105, Nigeria 4 Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto 840213, Nigeria 5 Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria 810107, Nigeria * Author to whom correspondence should be addressed. † Presented at the 3rd International Electronic Conference on Processes—Green and Sustainable Process Engineering and Process Systems Engineering (ECP 2024), 29–31 May 2024; Available online: https://sciforum.net/event/ECP2024. Proceedings 2024, 105(1), 36; https://doi.org/10.3390/proceedings2024105036 Published: 28 May 2024 (This article belongs to the Proceedings of The 3rd International Electronic Conference on Processes—Green and Sustainable Process Engineering and Process Systems Engineering) Download keyboard_arrow_down Download PDF Download PDF with Cover Download XML Download Epub Download Supplementary Material Versions Notes Keywords: antiplasmodial property; Globimetula oreophila; natural product; resistance The development of parasite resistance to first-line antimalarial medicines, especially the ACTs, has made the research and development of novel antimalarial medications vital. Globimetula oreophila, a plant used in traditional medicine to treat malaria, is an example of a natural product that may provide new antimalarial drugs with fewer side effects, less drug resistance, and greater efficacy than synthetic drugs. This study aims to evaluate the antiplasmodial properties of G. oreophila's fractions. After collection and authentication, the leaves were air-dried and reduced in size using a pestle and mortar. The pulverized plant was macerated in 70% ethanol and fractionated with solvents in the increasing polarity of n-hexane, chloroform, ethyl acetate, and n-butanol to produce the various fractions. The antiplasmodial activity of the n-hexane, chloroform, ethyl acetate, and n-butanol fractions of Globimetula oreophila leaf extract was assessed using an in vivo method in Plasmodium berghei-infected mice. In the mice, the fractions' median fatal dose (LD50) was calculated to be more than 5000 mg/kg. At doses of 125, 250, and 500 mg/kg, the fractions significantly (p < 0.001) reduced the parasitemia level. The fractions of G. oreophila showed significant in vivo antiplasmodial activity, which upholds the earlier in vivo findings for the crude extract as well as its folkloric use. Supplementary MaterialsThe following are available online at https://www.mdpi.com/article/10.3390/proceedings2024105036/s1.Author ContributionsConceptualization, D.G., Y.M.S., M.G.M. and H.H.S.; methodology, D.G., Y.M.S., M.G.M. and H.H.S.; software, D.G., B.H.A. and B.B.; validation, D.G., A.S., M.G.M., Y.M.S. and H.H.S.; formal analysis, D.G., B.H.A., A.M.M., M.I.A., M.G.M. and Y.M.S.; investigation, D.G., Y.M.S., M.G.M., M.I.A. and H.H.S.; resources, D.G., A.S., B.B., M.G.M. and B.H.A.; writing—original draft preparation, D.G., Y.M.S., M.G.M. and H.H.S.; writing—review and editing, D.G., Y.M.S., M.G.M., H.H.S. and A.M.M.; supervision, Y.M.S., M.G.M. and H.H.S.; project administration, Y.M.S., M.G.M., H.H.S. and D.G. All authors have read and agreed to the published version of the manuscript.FundingThis research received no external funding.Institutional Review Board StatementThis study was conducted by the Declaration of Helsinki and approved by Ahmadu Bello University Zaria, Committee on Animal Use and Care (ABUCAUC) with an approval number: ABUCAUC/2023/061.Informed Consent StatementNot applicable.Data Availability StatementThe data presented in this study are available on request from the corresponding author. The data are not publicly available due to ethical concerns about plagiarism.Conflicts of InterestThe authors declare no conflicts of interest.Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style Garba, D.; Ali, B.H.; Bawa, B.; Sanusi, A.; Sani, Y.M.; Abdullahi, M.I.; Magaji, M.G.; Musa, A.M.; Sadiya, H.H. Therapeutic Potential of Fractions of Globimetula oreophila (Oliv. ex Hook.f.) Leaf Extract Growing on Azadirachta indica against Plasmodium berghei-Infected Mice: An In Vivo Approach. Proceedings 2024, 105, 36. https://doi.org/10.3390/proceedings2024105036 AMA Style Garba D, Ali BH, Bawa B, Sanusi A, Sani YM, Abdullahi MI, Magaji MG, Musa AM, Sadiya HH. Therapeutic Potential of Fractions of Globimetula oreophila (Oliv. ex Hook.f.) Leaf Extract Growing on Azadirachta indica against Plasmodium berghei-Infected Mice: An In Vivo Approach. Proceedings. 2024; 105(1):36. https://doi.org/10.3390/proceedings2024105036 Chicago/Turabian Style Garba, Dauda, Bila Hassan Ali, Bashar Bawa, Abdulrazaq Sanusi, Yahaya Mohammed Sani, Musa Isma'il Abdullahi, Muhammad Garba Magaji, Aliyu Muhammad Musa, and Hassan Halimatu Sadiya. 2024. "Therapeutic Potential of Fractions of Globimetula oreophila (Oliv. ex Hook.f.) Leaf Extract Growing on Azadirachta indica against Plasmodium berghei-Infected Mice: An In Vivo Approach" Proceedings 105, no. 1: 36. https://doi.org/10.3390/proceedings2024105036 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.