Abstract SI (Self-incompatibility) is one of the major obstructions for the development and propagation of inbred lines in most Brassica vegetable crops. The manipulation of SI genes is an effective way to create Self-compatible (SC) materials. A mutated pollen recognition specificity gene BnSP11-1 on the A genome was responsible for the self-compatibility of the B. napus (AACC) cultivar ‘Westar’. In this study, site-specific manipulation of stigma recognition specificity gene BnSRK-1 was carried out using the CRISPR/Cas9 system in ‘Westar’. Then the mutated SI genes (BnSP11-1 and BnSRK-1) were transferred to B.rapa by distant hybridization, by continuous back cross and self pollination, 3 elite and completely self-compatible lines were obtained. The mutated SI genes can be applied widely in Brassica vegetable crops with AA genome (2n=20), such as Chinese cabbage, Pak choi, Purple flowering stalks and Chinese flowering cabbage to accelerate the breeding process.
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Zhang et al. (2024) studied this question.
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