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We here introduce a novel combination of different methods, namely gas chromatography-mass spectrometry and RNAseq. The described method can be used to extract exocrine chemical compounds and RNA from the same individual. Using this protocol, metobolites like defensive secretions, pheromones, surface protectans and others can be linked to RNA-profiling. The protocol should be applicable for the majority of arachnids, insects and other arthropods.
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Fröhlich et al. (2024) studied this question.
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