Yanghanlia caeni gen. nov., sp. nov., a novel taxon within the family Alcaligenaceae isolated from sludge of a pesticide-manufacturing factory

A Gram-stain-negative bacterium, designated LG-2 T , was isolated from sludge collected at a pesticide-manufacturing factory in Jiangsu Province, PR China. Cells of strain LG-2 T were strictly aerobic, non-motile and spherical. Growth was observed at 15–42 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and 0–3.0 % (w/v) NaCl (optimum, 1.0 %). LG–2 T showed 95.5–96.9 % 16S rRNA sequence similarity to type strains in the genera Pusillimonas , Bordetella , Parapusillimonas , Candidimonas and Paracandidimonas of the family Alcaligenaceae . The phylogenomic tree indicated that strain LG-2 T was clustered in the family Alcaligenaceae and formed a clade with Paracandidimonas soli IMT-305 T , while the phylogenetic trees based on 16S rRNA gene sequences indicated that strain LG-2 T formed a distinct clade within the family Alcaligenaceae . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between LG-2 T and its closely related type strains in the genera Pusillimonas , Bordetella , Parapusillimonas , Candidimonas and Paracandidimonas were 70.8–75.3, 18.9–23.7 and 59.6 %–69.3 %, respectively. The major cellular fatty acids were C 16 : 0 , C 17 : 0 cyclo, summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and summed feature 2 (C 12 : 0 aldehyde and/or unknown 10.928). The predominant menaquinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two aminophospholipids, three aminolipids and nine unknown polar lipids. The genome size of strain LG-2 T was 3.2 Mb and the DNA G+C content was 63.4 mol%. On the basis of the phenotypic, phylogenetic and genomic results from this study, strain LG-2 T represents a novel species of a new genus in the family Alcaligenaceae , for which the name Yanghanlia caeni gen. nov., sp. nov. is proposed, with strain LG-2 T (=KCTC 8084 T = CCTCC AB 2023123 T ) as the type strain.