Simple workflow for high-titer microbial production of small antibodies

Abstract Increasing demand for biologics necessitates the rapid production of antibodies and their mimetics. Herein, we established a rapid workflow to develop a fermentation process for high-titer production of small antibodies using the yeast Komagataella phaffii. We screened yeast strains with the optimal gene copy number of target proteins and applied them to a feedback-controlled, high-cell density fed-batch fermentation, and achieved high-titer production of small antibodies with titers 3–7 g/L.