Quick protocol for protein extraction from adherent fish-derived fibroblasts v1

We present a rapid and efficient protocol for extracting total proteins from adherent fish-derived fibroblasts, specifically optimized for applications in Western blotting and mass spectrometry. The method involves directly dissociating cells from the culture flask into a lysis buffer (e.g. RIPA buffer) followed by centrifugation to collect the total soluble proteins. The use of buffers like RIPA ensures comprehensive solubilization of cellular proteins while preserving their integrity and functionality. This straightforward and reproducible protocol yields high-quality protein extracts suitable for various downstream analytical techniques. Its simplicity and reliability make it an invaluable tool for researchers studying proteomics using fish cell culture.