This protocol describes the steps to prepare liquid culture medium for Saccharomyces cerevisiae. This liquid medium is used to optimize yeast cultures for fluorescence imaging by the reduction of auto-fluorescence through an abundance of Adenine [1]. The surplus of Methionine in the medium represses the pp7-CP expression, which reduces the level of CP aggregates [2].
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Hammer et al. (2024) studied this question.
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