Fluorescence-activated nuclei sorting (FANS) for single-cell Whole Genome Sequencing (scWGS) v1

This is the authors' abstract. We don't add key points for this paper.

This protocol describes the isolation steps of nuclei from human post-mortem brain samples, immunofluorescence, and nuclei sorting (FANS) for low coverage (<1x) single-cell Whole Genome Sequencing (scWGS) to detect mega-base somatic Copy Number Variations (CNVs). We have used this protocol to isolate nuclei from the frontal cortex, cingulate cortex, and substantia nigra tissues. However, it can be adapted for nuclei from different body areas, cell culture materials, and/or samples from other species.