Key points are not available for this paper at this time.
This protocol describes isolation of nuclei from frozen tissue using gentleMACS homogenization and FANS. Nuclei are permeabilized, washed, and counted; single-nucleus suspensions of sufficient concentration and nuclei quality may then be processed using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (CG000338, Rev F) protocol from 10X Genomics.
ereisner not provided (Sat,) studied this question.