This method presents an optimized protocol for the automated extraction of environmental DNA (eDNA) from estuarine samples using magnetic beads. The addition of Proteinase K to the lysis solution facilitates DNA solubilization at 56°C and mechanical rotation ensures thorough lysis. The DNA is then purified using a double ethanol wash and magnetic bead-based extraction on a KingFisher system. The method is designed for filters preserved in Longmire's buffer. If a different preservation is used the first step should be modified.
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Baidouri et al. (2024) studied this question.
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