Melatonin Block Tramadol-Induced Conditioned Place Preference in Rats: Implications for Addiction Treatment

Abstract ID 92087 Poster Board 318 Melatonin, a hormone regulating the sleep-wake cycle and various physiological functions, has shown promise in treating conditions such as sleep disorders, depression, and addiction. Tramadol, a widely prescribed opioid analgesic, has a great risk of addiction. This study investigates the potential of melatonin to block the rewarding effects of tramadol in a conditioned place preference. Male Wistar rats were classified into 4 groups 1) control 2) tramadol 3) tramadol + melatonin (one dose), and 4) tramadol + melatonin (multiple doses). The conditioned place preference test evaluated the animals' preference for chambers associated with tramadol effects. Behavioral parameters were analyzed using two-way ANOVA with Tukey's post hoc test. Tramadol administration induced a significant increase in conditioned place preference compared to the control group. A single dose of melatonin attenuated tramadol-induced conditioned place preference, while repeated doses did not show a significant effect. Additional analyses revealed that melatonin had no significant impact on total activity, ambulatory count, resting time, or distance traveled during the conditioned place preference test. This study shows the potential of melatonin in blocking tramadol-induced conditioned place preference, suggesting its therapeutic role in addressing tramadol addiction. Single-dose melatonin administration showed great efficacy, emphasizing its relevance in preventing the rewarding effects of tramadol.