FIGURE 6 from Exercise-induced β2-adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded γδ T-Cells via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

Interactions between DNAM-1 and PVR/Nectin-2 enhances the antileukemic activity of exercise expanded Vγ9Vδ2+ T-cells. A, Illustration of the receptors expressed on Vγ9Vδ2+ T-cells and their corresponding ligands expressed on cancer cells. B, Heat map of the surface expression and MFI of ligands and receptors on K562 cells. C and D, The specific lysis of K562 cells by expanded Vγ9Vδ2+ T-cells under two conditions: unstained control (−) and respective blocking antibody (+), DNAM-1, PVR, Nectin-2, TRAIL, TRAIL-R1, and TRAIL-R2 (n = 3). Significant differences between the between the unstained (−) and blocking conditions (+) were indicated by * and #, respectively. Data are represented as mean ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by repeated measures two-way ANOVA with Bonferroni post hoc test (C–D).