FIGURE 5 from Exercise-induced β2-adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded γδ T-Cells via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

Systemic β-AR activation through either acute exercise or ISO infusion evoke similar transcriptomic and surface phenotype shifts among ex vivo expanded Vγ9Vδ2+ T-cells. A, The percentage of activating, inhibitory, and chemokine receptors among expanded Vγ9Vδ2+ T-cells before (REST) and during (EX) exercise or ISO infusion. (n = 8). Statistical significance set as *, P < 0.05. GSEA performed using KEGG and GO terms showing enriched pathways on expanded Vγ9Vδ2+ T-cells that were expanded after (B) exercise or (C) ISO infusion. Graphs show enriched upregulated (blue) and downregulated (red) pathways as well as the percentage of DEGs contributing to each mechanism. Statistical significance set as FDR ≤ 0.25. Data are represented as mean.