Association Between Dairy Cow Mastitis Causative Agent and Some Production Parameters using RT-PCR

Mastitis is the term used to describe inflammation in one or more udder parts in dairy cows, which are mostly brought on by bacterial infections that travel within the teat canal; the advancement of microscopes made it possible to identify the microorganisms that cause the majority of mastitis cases, and the process continues until the PCR. The aim of this work is to use RT-PCR to illustrate the association between the mastitis causative agent and some production parameters. In the first few months of 2019, 83 milk samples were aseptically obtained from the herd of 9,000 Holstein milking cows from cows that had been clinically confirmed to have mastitis. To perform the RT-PCR and DNA extraction for each sample, the King Fisher™ Duo Prime Purification System, the Mag MAX™ CORE Nucleic Acid Purification Kit, and the VetMAX™ Masti Type Kit were used. All data were analyzed using the Chi-square test to highlight the association between the causative agent of mastitis and some production parameters. The results indicated a significant association between mastitis and the infected quarter (χ2= 5.06, P=0.024) and between mastitis frequency of previous mastitis infections (χ2= 6.58, P=0.01), lactation number (χ2= 12.55, P=0.01), gestation period (χ2= 32.97, P=0.01), and cow age (χ2= 15.88, P=0.01). Since there are many possible infections and its complicated etiology makes controlling mastitis difficult, more work needs to be put into managing the milking and milking parlor.