The State of Pro- And Antioxidant Balance of Rats’ Blood Under Condition of Combined Caffeine and Ethanol Intake and Melatonin Administration

The аim of research – to investigate prooxidant and antioxidant parameters in the bloodof rats subjected to alcohol intoxication, caffeine and melatonin administration.Materials and methods. Experiments were performed on 30 male rats weighing 180-200g. Subacute alcohol intoxication was induced by intragastric administration of 40 %ethanol at a dose of 7 ml/kg of the body weight for 7 days. Caffeine was administeredby gavage at a dose of 30 mg/kg of the body weight. The content of malondialdehyde inerythrocytes, oxidatively modified proteins, SH-groups, ceruloplasmin, catalase activityin blood plasma was determined.Results. It was revealed that alcohol intoxication increased malonic dialdehyde andoxidatively modified proteins content 40 % and 37.5 %, respectively. Сo-administrationof ethanol with caffeine reduced the intensity of oxidative stress in the blood. Ratsexposed to ethanol and its combination with caffeine showed decrease in SH-groupsin the blood 25 % and 39.7 % below control. Ceruloplasmin and catalase levels weremarkedly elevated in alcohol- exposed rats besides that catalase activity further increasedwith the combined ethanol and caffeine exposure. Administration of 5 mg/kg melatoninfor 7 days effectively normalized prooxidant and antioxidant markers in ethanol- exposedrats. However, in case of ethanol and caffeine intake, melatonin’s impact on catalase andceruloplasmin levels was less markable.Conclusions. Thus, melatonin showed antioxidant properties, decreasing alcohol- inducedoxidative stress in rats’ blood, but its impact was reduced in the presence of caffeine.