Protective effect of pectolinarin against H₂O₂-induced oxidative stress in C6 glial cells

This study aims to investigate the protective effects and mechanisms of pectolinarin against hydrogen peroxide (H 2 O 2 ) induced oxidative stress in C6 glial cells.Neurodegenerative diseases are potentially associated with oxidative stress, which causes excessive production of reactive oxygen species (ROS) that damage DNA and proteins in glial cells.The results of this study demonstrate that pectolinarin significantly increased cell viability, reduced ROS production, and Lactate dehydrogenase release in the H 2 O 2 -induced C6 glial cells.Additionally, pectolinarin decreased the level of pro-inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha from H 2 O 2altered levels back close to normal C6 cell levels.These findings suggest that pectolinarin has the potential to be used as a natural material to be applied in the treatment of neurodegenerative diseases by mitigating oxidative stress-induced damage to C6 cells.