CRISPR-Cas systems are activated by the formation of a ribonucleoprotein and its binding to the target nucleic acid sequence. Both binding processes are important for the overall performance. In their Communication (e202404069) Hongquan Zhang, X. Chris Le et al. describe a new method to study the binding kinetics and report the rate constants (kon and koff) and dissociation constant (Kd) for the activation of Cas13a by its RNA target. The method can unravel and quantify the kinetics of activation and enzymatic cleavage in a dynamic system. Artwork created by Jeffrey Tao.
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Feng et al. (2024) studied this question.
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