Tracking the dynamics of genomic loci is very important to know genome organization and gene function regulation. However, tools for imaging genomes, especially non-repetitive locus have still been limited. This protocol uses endonuclease-dead, programmable RNA-guided DNA-targeting Cas12 DNases (d)Cas13 proteins fused with fluorescent proteins to visualize and track DNA dynamics in live cells. This protocol details several aspects of the procedure, including gRNA design, CRISPR array construction and imaging.
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Yang et al. (2024) studied this question.
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