InVitro research of the neutrophil elastase inhibitory effects on the bacterias Pseudomonas aeruginosa and Klebsiella pneumoniae

Numerous findings highlight neutrophil elastase's (NE) antibacterial role in human innate immunity, synthesized and secreted by neutrophils during inflammation through the ELANE gene expression.Some clinically significant bacteria, such as Pseudomonas aeruginosa and Klebsiella pneumoniae, due to induction of some specific genes over-expression, possess a powerful potential for developing the adaptive resistance to a large antibiotics number (multi-drug resistance).Therefore, in our in vitro research, the influence of different NE concentrations (NE1 and NE2) on the bacterial growth, their total proteins, as well as the bacterial protein virulence factor leucine aminopeptidase (LAP) excretion.Also, the obtained results showed NE