Assessing the Effectiveness of Xylene-Free Deparaffinization in Hematoxylin and Eosin Staining

Xylene is the deparaffinizing agent used in Hematoxylin and Eosin staining. It is expensive, dangerous, and will contaminate the workplace. There's also the disposal issue. Therefore, using a low-cost and environment friendly deparaffinization replacement is highly beneficial. To assess how well xylene-free sections perform in terms of yielding sufficient H and E staining in a straightforward, economical, and faster manner in comparison to traditional H and E sections, forty paraffin blocks were taken into account, a piece was stained using the traditional H and E procedure and another using the H and E method without xylene. Slides were graded according to the following criteria: uniformity, clarity, crispness, nuclear and cytoplasmic staining.