FIGURE 3 from De Novo Purine Metabolism is a Metabolic Vulnerability of Cancers with Low p16 Expression

p16/CDKN2Alow cells are more sensitive to inhibitors of nucleotide metabolism. A, Table of inhibitors used in in vitro cell line studies. 1C metabolism = one carbon metabolism. B, SKMEL28 human melanoma cells were infected with lentivirus expressing a shRNA targeting p16 (shp16). shGFP was used as a control (shCont). Cells were treated with the indicated inhibitors and proliferation was assessed by crystal violet staining. IC₅₀ and fold change (shp16 vs. shCont, FC) are indicated. Data from one of 2–3 independent experimental replicates are shown (n = 6). C, Increased drug sensitivity from DepMap data of cutaneous melanoma cell lines with high or low CDKN2A expression. Data are mean ± SD. t test. *, P < 0.05; **, P < 0.01.