Supplementary Figure S1 from Machine Learning Links T-cell Function and Spatial Localization to Neoadjuvant Immunotherapy and Clinical Outcome in Pancreatic Cancer

Supplementary Figure S1. A. Stacked bar chart showing percent tissue area (out of 100) sampled per resection from each patient. Bars are colored by histopathologic site of the regions sampled. B. Representative IHC staining of each antibody used in sequence in the panel. Scale bar = 50 μm. C. Two representative regions stained with CD3, CD8, and CD4 antibodies. For each region, top images show gates for CD8 on CD3+ population (left) and CD4 on CD3+ CD8- population (right), and bottom row shows pseudo-colored mIHC images. D. Hierarchical gating template used to phenotype cells using image gating cytometry in FCS Image Cytometry RUO.