Supplementary Figure S1 from Radiotherapy Enhances Metastasis Through Immune Suppression by Inducing PD-L1 and MDSC in Distal Sites

Supplementary figure 1. Radiation is not able to induce an abscopal effect. A. Growth of LLC flank local tumors received local RT. B. Flow cytometry result of whole lung gated on CD45- CD31- population. C. q-PCR result indicating expression of genes in sorted CD45- CD31- lung cells (Mets), with LLC and MC38 cell lines as controls. The relative expression is presented as fold over those of LLC. D. Quantification of lung metastasis of LLC tumors in the MC38/LLC tumor model by percentage of tumor area. E. Growth of heterogeneous 2-tumor model (MC38/B16F10) in which MC38 received ctrl (sham treatment) or IR. F. Growth curve of the local KPC tumors treated with control and 20 Gy. G. Distal LLC tumors growth curve. H. Number of LLC lung mets in control and irradiated mice in KPC/LLC model. I. Percentage of metastasis area of total lung. J. Representative lung sections of the mice treated with control or IR. Statistics by ANOVA (A, E, F, G) and t-test assuming unequal variance (B, D, H, G). A and E, N=5. Experiments were repeated 3 times. F-I, N=6-7