Median lethal dose (LD50) estimation of Cypermethrin and Profenofos in Swiss Albino mice (Mus musculus)

Present study was undertaken to determine the Oral median lethal dose (LD50) of Cypermethrin, a pyrethroid and Profenofos, an organophosphate pesticide in male and female Swiss albino mice. The selected doses of Cypermethrin based on the pilot study were 200, 225, 250, 275 and 300 mg/kg B.W. for male and 350, 375, 400, 425 and 450 mg/kg B.W for female animals. Corresponding doses of Profenofos based on the pilot study was found to be 10, 15, 20, 25 and 30 mg/kg B.W for male and 20, 25, 30, 35 and 40mg/kg B.W for female. Pesticide was dissolved in corn oil and administered orally to different groups of male and female mice, consisting of 10 animals in each group. Possible death of animals was monitored up to 96 h to calculate the median lethal dose (LD50) of CYP and PFF. The results obtained in this study suggest that oral LD50 of the CYP and PFF dissolved in corn oil was found to be (249.06 ±30.75, 406.38 ± 64.28)and (24.26 ±6.43, 39.67 ±6.2) mg/kg B.W. respectively in male and female Swiss albino mice.