Detection of AMPC Βeta lactamase Producing Acinetobacter Species with Their Antibiotic Resistance Profile in Tertiary Care Hospital

Due to the increased use of broad-spectrum antibiotics in hospitalised patients, Acinetobacter species has become a prominent multi drug resistant nosocomial pathogen being able to colonize and develop infections in patients.Acinetobacter species has numerous mechanisms for resistance, including an impermeable outer membrane, enzymes that break down antibiotics, particularly AmpC, Metallo β-lactamases, class D OXA-type and class B Metallo β-lactamases that allow the organism to resist carbapenems, porin channel alterations as well as efflux pumps, and other genetic changes.It is well known that AmpC β-lactamases confer resistance to cephalosporins in the oxyimino group and are unaffected by currently available β-lactamase inhibitors.The aim of the study was to detect AmpC β-Lactamase in Acinetobacter species with their Antibiotic Resistance pattern.100 Acinetobacter species isolates from various clinically relevant samples were included.Methods such as the Disc Approximation test and the Modified 3-Dimensional test were employed on ceftazidime resistant isolates of Acinetobacter species to detect AmpC β-lactamase phenotypically.Highest prevalence of Acinetobacter was found in Blood (37%).Maximum number of Acinetobacter were isolated from MICU.The Modified 3-Dimensional produced the largest percentage of AmpC positive Acinetobacter isolates (40.67%) compared to the Disc Approximation Test method (33.89%).Early detection of these β-lactamases production is essential for planning appropriate medication in accordance with the resistance mechanisms of the MDR strains, as well as for epidemiological research and efficient infection control procedures to prevent the spread of infection.