Simultaneous Estimation of Metoclopramide Hydrochloride and Dexamethasone Sodium Phosphate Using Area Under Curve Method

The objective of the study is to develop simple, precise, authentic and cost-effective analytical method for the estimation of antiemetic drugs according to ICH guidelines.The working standard solutions of MET and DEX were scanned in UV from the range of 200-400 nm where the λmax or the absorption maximum of metoclopramide hydrochloride and dexamethasone sodium phosphate was 273 nm and 241.60 are respectively.The wavelength range selected for metoclopramide was 292-320 (λ1-λ2) nm and for dexamethasone sodium it was 219-256 (λ3-λ4) nm.Developed methods are validated as per ICH guidelines.The UV method was developed by using 8-16µg/ml of MET.HCl and 6-12µg/ml DEX.Area under curve method was developed.The correlation coefficient was found to be 0.9946 for MET.HCl and 0.9985 for DEX.