Spectroscopic Study of β-carotene

The spectroscopic study of bita carotene has been performed experimentally. A simple, rapid and cost efficient method has been utilized to extract required chemical (bita carotene) from natural carrot. Ethanol was used as organic solvent, water for the phase seperation and petroleum ether were used for purification purpose. This paper has aimed to get information related to electronic transition in some part of ultraviolet to visible region of electromagnetic spectrum. For observing absorption spectra, 400 nm to 500 nm excitatation wavelength were used in PC based double beam UV- Visible spectrometer. In analysis part of naturally extracted bita carotene from fresh carrot, we observe that conjugated chain in carotenoids absorbs visible (blue/green) part of electromagnetic spectrum and that is why Beta-carotene appears red-yellow as yellow colour get reflected back to us. And hence it has also been obtained that this symmetric molecule is a fluorescent chromophoric biomolecule. People have studied Raman spectra of bita carotene where in spectrum great enhancement has been observed in Raman signal in electronic transition region due to S0 to S2 transition which strongly support our result that bita carotene is a fluorescent chromophoric biomolecule having maximum electronic transition in visible (blue/green) region so appears yellowish red.