April 27, 2024Open Access

An inflection point in high-throughput proteomics with Orbitrap Astral: analysis of biofluids, cells, and tissues

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Authors

NHNathan G. HendricksUniversity of California, Riverside SBSantosh D. BhosaleCedars-Sinai Medical Center AKAngel J. KeoseyanCedars-Sinai Medical Center

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Implication

Technical note demonstrates robust high-throughput proteome profiling across diverse biofluids and tissues, highlighting scalable workflows for clinical biomarker discovery.

Key Points

High-throughput proteomics profiling resolves over 10,000 proteins across human cells and multiple tissues within a rapid 24-minute active gradient.
More than 80% of quantified proteins achieve a CV below 20% across replicate injections, confirming high quantitative reproducibility for Orbitrap Astral runs.
Assessment using automated SP3 protein digestion and adaptive focused acoustics streamlines sample handling, which may enable rapid translational biomarker discovery.

Cite This Study

Hendricks et al. (2024) studied this question.

synapsesocial.com/papers/68e6d425b6db64358765195f https://doi.org/10.1101/2024.04.26.591396

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Implication

Technical note demonstrates robust high-throughput proteome profiling across diverse biofluids and tissues, highlighting scalable workflows for clinical biomarker discovery.

An inflection point in high-throughput proteomics with Orbitrap Astral: analysis of biofluids, cells, and tissues

Discussion

Member takes

Implication

Key Points

Cite This Study

An inflection point in high-throughput proteomics with Orbitrap Astral: analysis of biofluids, cells, and tissues

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider