Primary Stage of Toxocara Antigen Production

The article describes a method for isolating Toxocara canis larvae from chorions. Nematode larvae can serve as a specific antigen in the diagnosis with serological reactions. The study material was cultures of Toxocara eggs obtained from female nematode gonads. Toxocara eggs were stored in a 4.0% formalin solution or a 1.0% hydrochloric acid solution. Sexually mature female Toxocara species were obtained by isolating helminths from the intestines of carcasses of naturally infected carnivores. For artificial isolation of Toxocara larvae from eggs, a method of their short-term incubation in the bile of ruminants was used. The nematode egg culture was put on a heated magnetic stirrer. A magnet in plastic of 1.5–3.5 cm was placed in a sample, and heating to 38±1 оC was turned on. After 30-minute exposure, the larvae were washed in the saline. For convenience, they may be placed in a Berman device at 38±1 оС. The materials freshly obtained and stored for 3 months showed a high percentage of larval yield from eggs. The stored material showed a rather worse result for the 1st year.