Using spectral derivatives to remove the influence of hair on optical images of the static absorbing properties of tissue-like turbid media

SignificanceAlthough measurements of near-infrared light diffusely reflected from the head and other biological tissues are commonly used to generate images revealing changes in the concentrations of oxy- and deoxy-hemoglobin, static imaging of absolute concentrations has been inhibited by the unknown and variable coupling between the optical probe and the skin, to which hair is often a significant contributor. Measurements of spectral derivatives provide a means of overcoming this shortcoming.AimThe aim is to demonstrate experimentally that measurements of the derivative of the attenuation of the detected signal with respect to wavelength can be used to achieve images that are immune to the spatial variation of hair on the surface. The objective is to generate topographic images representative of static absorbing properties rather than retrieving absolute optical coefficients, which requires a tomographic approach.ApproachThe surface of a tissue-equivalent phantom, containing targets with different concentrations of absorbing dye, was coated with a layer of dark hair. The phantom was then imaged using a broadband source and spectrometer, and prior knowledge of the absorbing characteristics of the dye and of melanin was used to acquire separate images of each.ResultsThe targets within the phantom are revealed with remarkable clarity, although a nonlinear relationship between the target contrast and absorption was observed. This nonlinear behavior was confirmed and explained using a Monte Carlo model of light propagation in a slab of similar absorbing properties.ConclusionsA spectral derivative approach could be an effective tool for in vivo topographic imaging of the static optical properties of the brain and other tissues, avoiding the deleterious effects of hair.