Preparation and Preliminary Application of Fluorescent Microsphere Test Strips for Feline Parvovirus Antibodies

Abstract This study introduces a novel diagnostic modality for the detection of feline panleukopenia virus (FPV) antibodies in feline serum by using fluorescent microsphere immunochromatographic test strips (FM-ICTS). Leveraging the inherent specificity of antigen-antibody interactions, the FM-ICTS approach demonstrates considerable potential for efficient and accurate FPV antibody detection within a short timeframe. Through rigorous sensitivity and specificity assessments, the method exhibits commendable diagnostic performance, exhibiting favourable accuracy and stability characteristics over an extended period. The clinical validation involving 84 samples underscores the reliability of the test by corroborating its outcomes with established haemagglutination inhibition (HI) assays. This innovative FM-ICTS framework emerges as a promising avenue for expedient and dependable disease diagnosis within the realm of veterinary science, offering implications for timely disease management and surveillance.