Multimodal imaging of head and neck tumors in the chorioallantoic membrane assay as a basis for the establishment of new oncological therapies

Introduction Head and Neck Tumors (HNTs) exhibit poor prognosis due to their heterogeneity. The Chorioallantoic Membrane (CAM) Assay, established as a bridge between in vivo and in vitro research, is limited by inadequate standardization and diverse evaluation approaches. This study aimed to establish reproducible high engraftment rates and multimodal imaging: photography, sonography, PET-CT, MRI, autofluorescence measurement, intravital fluorescence microscopy (IVM), and histology.