In vitro anti-cancer activity of Tetraponera rufonigra extract on breast cancer cell line MB-231

Abstract This study explores the medicinal value of Tetraponera rufonigra, a unique ant species found in Asia and Africa. Specifically, we investigate its potential in producing anticancer drugs within the fascinating realm of ethnoentomology. Tetraponera ants produce neurotoxic substances called tetraponerines, which have demonstrated cytotoxic effects on cancer cells, including MCF-7 breast carcinoma and colon HT29 cells. Our findings suggest that these ants could be a valuable source of new anticancer drugs, shedding light on the rich ethnoentomological relationship between humans and insects. The methodology involved ethnoentomological data collection, material selection, authentication, extract preparation, and biochemical screening. We used the MB231 breast cancer cell line for in vitro testing. The results revealed significant cytotoxicity against breast cancer cells, with an IC50 value of 760 µg/ml. Further research is needed to validate its efficacy and identify the active compounds. This study not only contributes to the field of cancer research but also emphasizes the importance of ethnoentomological knowledge in understanding the potential of insect-derived compounds for cancer treatment.