Proliferation of psychrotrophic bacteria in cold‐stored platelet concentrates

Background and Objectives Platelet concentrates (PC) are stored at 20–24°C to maintain platelet functionality, which may promote growth of contaminant bacteria. Alternatively, cold storage of PC limits bacterial growth; however, data related to proliferation of psychotrophic species in cold‐stored PC (CSP) are scarce, which is addressed in this study. Materials and Methods Eight laboratories participated in this study with a pool/split approach. Two split PC units were spiked with ~25 colony forming units (CFU)/PC of Staphylococcus aureus , Klebsiella pneumoniae , Serratia liquefaciens , Pseudomonas fluorescens and Listeria monocytogenes . One unit was stored under agitation at 20–24°C/7 days while the second was stored at 1–6°C/no agitation for 21 days. PC were sampled periodically to determine bacterial loads. Five laboratories repeated the study with PC inoculated with lyophilized inocula (~30 CFU/mL) of S. aureus and K. pneumoniae . Results All species proliferated in PC stored at 20–24°C, reaching concentrations of ≤10 9 CFU/mL by day 7. Psychrotrophic P. fluorescens and S. liquefaciens proliferated in CSP to ~10 6 CFU/mL and ~10 5 CFU/mL on days 10 and 17 of storage, respectively, followed by L. monocytogenes , which reached ~10 2 CFU/mL on day 21. S. aureus and K. pneumoniae did not grow in CSP. Conclusion Psychrotrophic bacteria, which are relatively rare contaminants in PC, proliferated in CSP, with P. fluorescens reaching clinically significant levels (≥10 5 CFU/mL) before day 14 of storage. Cold storage reduces bacterial risk of PC to levels comparable with RBC units. Safety of CSP could be further improved by implementing bacterial detection systems or pathogen reduction technologies if storage is beyond 10 days.