Optimal culture of Bacillus altitudinis X-10 strain secreting enzyme cocktails for enhancing biomass saccharification of wheat straw

Developing an efficient and continuous biomass saccharification system provides significant insight into sustainable energy. In the present work, a constant and effective bacterial saccharification system was developed to degrade wheat straw (WS) into sugars. Newly identified Bacillus altitudinis X-10 was selected to optimize sugar release from wheat straw via Box-Behnken Design (BBD). The released reducing sugars were 38.5 mg/g under the optimal fermentation conditions of pH 6.98, a temperature of 29.5°C, and a solid-liquid ratio of 0.84%. In addition, B. altitudinis X-10 can produce low-cost enzyme cocktails, which contain remarkable xylanase activity (2.76 ± 0.19 U/ml). The enzyme cocktail can break down untreated wheat straw biomasses and serve as a pretreatment to enhance the ability of cellulase to degrade wheat straw. The saccharification system can increase wheat straw digestibility, leading to a cost-effective and continuous bioconversion strategy for renewable energy and bio-based industries.