Tissue Fixation | HubMAP | JHU-TMC v2

First and most important - the original tissue sample must be of good quality. Factors such as warm ischemic time, the time delay between tissue excision and fixation, etc. are important. Ideally, tissues should be acquired as close to still being viable as possible, and put into fixative as soon as possiblefollowing excision. Delays lead to cell death, autolysis, and loss of tissue and cell integrity with concomitant losses of immunostaining (e.g., due to proteolysis of the antigen). If acquiring animal tissues, consider performing perfusion fixation before organ/tissue removal if it is an option