Microfluidic System with Integrated Electrode Array for High-Throughput Electrochemical Impedance Spectroscopy Analysis of Localised Cells

A multi-channel microfluidic system was designed and fabricated with an integrated electrode array to be capable of trapping and analysing single cells or populations in the individual channels in a controlled chemical environment. The analytical system was interfaced with a dedicated printed circuit board designed for parallel EIS and optical screening. The continuous impedance measurement also monitors the adequate filling of the channels and the cell trapping events. Yeast cells were characterised by recording the EIS spectra of individual channels, allowing the differentiation between the populations considering their size, viability, or proliferation. The EIS analysis was supported by fluorescent optical microscopy, also using cell staining.