Mp59-04 Micturition-Related Neural Activity in the Cerebral Cortex Depends on the Location, Cell-Type, and Projection Pathway Uncovered by Two-Photon Calcium Imaging

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP59)1 May 2024MP59-04 MICTURITION-RELATED NEURAL ACTIVITY IN THE CEREBRAL CORTEX DEPENDS ON THE LOCATION, CELL-TYPE, AND PROJECTION PATHWAY UNCOVERED BY TWO-PHOTON CALCIUM IMAGING Hiroshi Shimura, Satoshi Manita, Takanori Mochizuki, Masayuki Takeda, Kazuo Kitamura, and Takahiko Mitsui Hiroshi ShimuraHiroshi Shimura , Satoshi ManitaSatoshi Manita , Takanori MochizukiTakanori Mochizuki , Masayuki TakedaMasayuki Takeda , Kazuo KitamuraKazuo Kitamura , and Takahiko MitsuiTakahiko Mitsui View All Author Informationhttps://doi.org/10.1097/01.JU.0001009476.18935.3b.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The mechanism of micturition is complicated and especially in the cerebral cortex, micturition-related neural activity remains poorly understood. Two-photon calcium imaging enables to observe individual neural activities with high spatial resolution. In the present study, we observed the neural activities in the cerebral cortex using two-photon calcium imaging and evaluated the relationship with micturition. Our purpose was to reveal the ratio and the localization of micturition-related neurons, and the features of neural activity in various cell-type patterns. METHODS: We genetically expressed red calcium indicators in the anterior cingulate cortex (ACC) or the primary motor cortex (M1) of mice. Cell-type patterns were 1) non-selective neurons, 2) layer 5 pyramidal neurons, and 3) certain projection neurons; the ACC to the periaqueductal gray matter (PAG) or the M1 to the pontine micturition center (PMC). Two-photon calcium imaging from the ACC or the M1 was performed with bladder perfusion under urethane anesthesia. Micturition-related neurons were extracted according to neural synchrony with micturition. RESULTS: The rates of micturition-related neurons per all observed neurons were 1) 6.34%, 2) 10.97%, 3) 9.42% in the ACC and 1) 6.61%, 2) 10.33%, 3) 8.50% in the M1.The hot spot (high density region) of micturition-related neurons in the ACC (posterior and deep region) were more local than in the M1. The peak timing histogram of 2) activities in the ACC was bimodal (the former mean; 0.72 sec, the latter mean; 11.53 sec), and the latter peak was analogous with histogram of 3) activities (mean 10.75 sec). Furthermore, the population of the delayed neurons was located in the hot spot of the ACC (posterior and deep region). On the other hand, the patterns of neural activity were uniform among each variation in the M1. CONCLUSIONS: We represented that micturition-related neural activities in the cerebral cortex could be detected individually, which was the first study using two-photon calcium imaging for micturition. Especially in the ACC, the patterns of the activities depend on the cell-type and projection pathway, indicating that the presence of functional clusters. The various neural activity timings would indicate that these neurons play different roles for micturition each other. Utilizing this calcium imaging method would uncover the mechanism of micturition in the future. Source of Funding: JSPS KAKENHI Grant Number 20K18135, JP22K09496, Grant for Young Researcher from Yamanashi Prefecture, Young Research 108 Grant from the Japanese Urological Association,GSK Japan Research Grant 2021 © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e958 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Hiroshi Shimura More articles by this author Satoshi Manita More articles by this author Takanori Mochizuki More articles by this author Masayuki Takeda More articles by this author Kazuo Kitamura More articles by this author Takahiko Mitsui More articles by this author Expand All Advertisement PDF downloadLoading ...