Mp59-02 Imidazoline 2 Receptor Activation Improves Detrusor Overactivity of Mice With Spinal Cord Injury Through Relaxation of Bladder Smooth Muscles

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP59)1 May 2024MP59-02 IMIDAZOLINE 2 RECEPTOR ACTIVATION IMPROVES DETRUSOR OVERACTIVITY OF MICE WITH SPINAL CORD INJURY THROUGH RELAXATION OF BLADDER SMOOTH MUSCLES Mamoru Hashimoto, Kanako Matsuoka, Kang Jun Cho, Stephanie L. Daugherty, Nobutaka Shimizu, Kazutoshi Fujita, Akihide Hirayama, Hirotsugu Uemura, Sergei Karnup, and Naoki Yoshimura Mamoru HashimotoMamoru Hashimoto , Kanako MatsuokaKanako Matsuoka , Kang Jun ChoKang Jun Cho , Stephanie L. DaughertyStephanie L. Daugherty , Nobutaka ShimizuNobutaka Shimizu , Kazutoshi FujitaKazutoshi Fujita , Akihide HirayamaAkihide Hirayama , Hirotsugu UemuraHirotsugu Uemura , Sergei KarnupSergei Karnup , and Naoki YoshimuraNaoki Yoshimura View All Author Informationhttps://doi.org/10.1097/01.JU.0001009476.18935.3b.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To investigate the effects of imidazoline 2 receptor (I2R) activation on lower urinary tract dysfunction (LUTD) in mice with spinal cord injury (SCI). METHODS: C57BL/6N female mice at 9-10-weeks old were used. SCI mice underwent complete transection of the Th8-9 spinal cord. Four weeks after SCI, cystometrograms was performed under an awake condition. Two-weeks treatment with 2BFI (I2R agonist)was initiated at 2 weeks after SCI. In organ bath studies, the urinary bladder from SCI mice was cut into two longitudinal strips. Bladder body muscle strips were then mounted longitudinally in a vertical double-jacketed organ bath with 37 °C oxygenated 15 mL Krebs solution. BU224 (100 μM) (I2R antagonist) was applied to each bath 15 minutes prior to 2BFI application (1, 10, 100 μM). To induce precontraction of muscle strips, we added KCl (80 μM) or carbachol (10 μM) to each bath. We waited for 30-40 minutes after inducing precontraction before each drug was added. RESULTS: SCI mice with and without 2BFI exhibited significantly reduced voiding efficiency and an increased number of non-voiding contractions (NVC) compared with spinal intact mice. However, SCI mice with 2BFI showed better voiding efficiency and the reduced number of NVC than SCI mice without 2BFI. In organ bath studies, 2BFI induced relaxation in muscle strips precontracted with KCl or carbachol. BU224 reduced the relaxation effects of 2BFI (Figure 1). CONCLUSIONS: These results suggest that imidazoline 2 receptor activation improved detrusor overactivity evident as the NVC reduction, at least in part, by bladder smooth muscle relaxation in SCI mice. Thus, I2R could be a therapeutic target for the treatment of SCI-induced LUTD. Download PPT Source of Funding: Funding: NIH R01DK129194 © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e957 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Mamoru Hashimoto More articles by this author Kanako Matsuoka More articles by this author Kang Jun Cho More articles by this author Stephanie L. Daugherty More articles by this author Nobutaka Shimizu More articles by this author Kazutoshi Fujita More articles by this author Akihide Hirayama More articles by this author Hirotsugu Uemura More articles by this author Sergei Karnup More articles by this author Naoki Yoshimura More articles by this author Expand All Advertisement PDF downloadLoading ...