Pd54-02 Statin Ameliorates Ischemia-Induced Overactive Bladder With Improvement of Blood Flow and Anti-Inflammatory Effect in Rats

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Overactive Bladder II (PD54)1 May 2024PD54-02 STATIN AMELIORATES ISCHEMIA-INDUCED OVERACTIVE BLADDER WITH IMPROVEMENT OF BLOOD FLOW AND ANTI-INFLAMMATORY EFFECT IN RATS Yuichi Ishizuka, Yohei Satake, Shingo Kimura, Shinichi Yamashita, Naoki Kawamorita, and Akihiro Ito Yuichi IshizukaYuichi Ishizuka , Yohei SatakeYohei Satake , Shingo KimuraShingo Kimura , Shinichi YamashitaShinichi Yamashita , Naoki KawamoritaNaoki Kawamorita , and Akihiro ItoAkihiro Ito View All Author Informationhttps://doi.org/10.1097/01.JU.0001008724.35538.9c.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Statins are widely used to treat dyslipidemia and have been shown to reduce the risk of ischemic heart disease and cerebrovascular disease. The effects of statins on ischemia-induced overactive bladder (OAB) and the associated mechanisms were investigated in a rat model of chronic pelvic ischemia. METHODS: A pelvic ischemia model was created by iliac arterial injury (AI) and a high-fat diet using male Sprague-Dawley rats. Rats were assigned to 3 groups: the control group, AI group, and AI+statin group. The control group underwent sham operation and was fed a normal diet. The AI group underwent AI surgery and was fed a high-cholesterol diet. The AI+statin group was administered a simvastatin orally at a dose of 20 mg/kg once daily for 4 weeks. Cystometry was performed for 8 weeks after surgery. Bladder blood flow was evaluated by laser meter. Thickness of the iliac arteries were measured and microvascular density in the bladder lamina propria was evaluated by immunostaining for CD31. Expressions of inflammatory cytokines in the bladder were measured by real-time PCR. RESULTS: Cystometry showed that the voiding interval was shorter and voided volume was decreased in the AI group compared to the control group. The AI+statin group showed longer voiding interval and larger voided volume than the AI group (Figure 1A, B). The AI group showed decreased bladder blood flow (Figure 1C), increased iliac arterial wall thickening and decreased microvascular density compared to the control group. Statin administration improved the blood flow (Figure 1C). Iliac arterial wall thickening was suppressed and microvascular density was increased by statin, although not significantly. Real-time PCR showed significantly higher inflammatory cytokines (IL-6, IL-8, and TNF-α) in the AI group than in the control group, and they were lower in the AI+statin group than in the AI group (Figure 1D). CONCLUSIONS: The present results suggest that AI and high fat diet caused bladder ischemia and OAB, then simvastatin improved ischemia-induced OAB. The mechanism of the effects involves improved bladder blood flow and decreased bladder inflammation. Download PPT Source of Funding: The Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant no. 20K09571) © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1143 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Yuichi Ishizuka More articles by this author Yohei Satake More articles by this author Shingo Kimura More articles by this author Shinichi Yamashita More articles by this author Naoki Kawamorita More articles by this author Akihiro Ito More articles by this author Expand All Advertisement PDF downloadLoading ...